Kuyt couldn't have ended his career on the back of a better moment as he netted a hat-trick to down Heracles 3-1 to seal Feyenoord's first Eredivisie title in 18-years.
"To me this feels like the right time to retire".
The club said Kuyt would remain with Feyenoord in another role.
Feyenoord technical director Martin Van Geel said: "We are unbelievably grateful for what Dirk has done over the past two years". I have had two fantastic years here since returning to Feyenoord, with this season's title as the absolute pinnacle.
In this September 15m 2016 file photo Feyenoord's team captain Dirk Kuyt applauds the supporters at the end of the Group A Europa League match between Feyenoord and Manchester United at the De Kuip stadium in Rotterdam, Netherlands. "With his attitude, professionalism and passion, he has added a huge amount in the changing room, on the field and off it".
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"We are therefore delighted that we can retain someone with his experience and setting the club". "I'm going to train Dirk and I can imagine that he'll follow me in due course".
After that storybook ending, not even the prospect of Champions League soccer next season was enough to entice Kuyt into trying to add another year to his long playing career.
Having begun his professional career at Utrecht, Kuyt joined Feyenoord in 2003 and won the KNVB Cup during a three-year spell. He went to play at Liverpool for six seasons before spending a further three at Fenerbahçe. The Netherlands' win over Brazil in the 2014 World Cup bronze medal match was the last time Kuyt featured in an global match.
A native of Katwijk aan Zee, Netherlands, Kuyt will walk away from the game with 104 appearances for his national team, including a 2010 runner-up finish to Spain at the FIFA World Cup.