It is also the largest overall supplier of financial support for the Palestinians.
While Trump administration officials have said they expected a "cooling off period" with the Palestinians, Trump's tweets Tuesday signaled the President has grown frustrated with Palestinians' refusal to partake in a US-led peace process in the wake of his Jerusalem decision.
In a pair of tweets, Trump said the Palestinians show "no appreciation or respect" to the United States for aid money given to Palestinian territories. Barghouti added that Washington is no longer part of the peace process due to Trump's position toward Israel.
This centers around President Trump's Jerusalem declaration, which declared the city as Israel's capital, and fueled massive worldwide protests, but nowhere so much as Palestine, which considers the eastern part of the city their own capital.
For their part the Palestinians have said they no longer consider the US a fair broker in peace talks because of the White House's stance on the holy city.
Last week, Haaretz reported that the Palestinian Authority is about to hand in the documents required for signing on to 22 global conventions and treaties, 18 of which are under United Nations auspices, in protest against the unilateral American recognition of Jerusalem as Israel's capital. "We're going to make sure they come to the table".
His comments came hours after Hanan Ashrawi, a member of the Palestinian National Council, said the Palestinians would not be "blackmailed" by United States threats.
Christian groups decry United States policy change on Salvadorans as Episcopalians offer support
As an example, he noted that in 2016, the country received 52,000 deportees from the United States and Mexico. Salvadorans were first granted temporary residency protections in 1990 amid El Salvador's brutal civil war .
Other events in Washington in recent months have been created to put the squeeze on the Palestinians and make them return to the negotiating table.
The United Nations Relief and Works Agency (UNWRA) that is now targeted by the U.S. provides education, health care, and social services to the Palestinian diaspora in Jordan, Lebanon, Syria, the Gaza Strip and the West Bank, including East Jerusalem. The U.S. has also pledged to provide $38 billion in military aid to Israel over ten years, starting in the next fiscal year.
On Monday, the Palestinian government had recalled its United States ambassador following Trump's controversial decision of recognising Jerusalem as the capital of Israel a few weeks ago.
The US gave $357m to the Palestinian Authority in 2016, according to The Times of Israel.
Earlier this week, the PA said it was recalling its envoy to the United States following Trump's decision to recognise Jerusalem as Israel's capital.
The U.S. now gives $300 million in funding to the agency.
Culture minister Miri Regev, a senior member of Israeli Prime Minister Benjamin Netanyahu's cabinet, said she was "very pleased" by Mr Trump's call to cut funding to the Palestinians.