Video screens played images of the Italy defender and the mascots wore either Fiorentina or Cagliari shirts - the latter being one of Astori's former clubs.
The game was halted in the 13th minute - a tribute to Astori's shirt number - as both sets of players let out a deafening round of applause and a tifo of "Davide 13" emblazoned the top tier of Stadio Artemio Franchi.
So who is the worst Premier League manager ever, Jose?
Analysing a performance for me is more than number of goals or assists, and for me I'm really happy with Marcus". He plays for United many matches, he starts some comes from the bench for others.
The game was paused in the 13th minute as the club's fans commemorated the Italy global with a stunning display.
Fiorentina held on to their one-goal lead to win the match and move up to ninth in the table.
During the last stages of the game, Benevento's Massimo Coda struck the post, with Fiorentina's new captain Milan Badelj also hitting the woodwork.